Epigenética Aplicada

Programa de Pós-Graduação Stricto Sensu em Patologia Ambiental e Experimental

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A disciplina visa introduzir os fundamentos teóricos e aplicação das principais técnicas de biologia molecular e ensaios bioquímicos aplicados ao estudo dos mecanismos Epigenéticos, bem como possibilitar os alunos para escolha da(s) técnica(s) mais adequadas para delineamento de projetos relacionados ao estudo dos mecanismos Epigenéticos.

Bibliografia:

BUENROSTRO, J. D.; WU, B.; CHANG, H. Y.; GREENLEAF, W. J.: ATAC-seq: a method for assaying chromatin accessibility genome-wide. Current Protocols in Molecular Biology, v. 109, p. 21.29.1-21.29.9, 2015. DOI: https://doi.org/10.1002/0471142727.mb2129s109.
GARRIDO-CARDENAS, J. A.; GARCIA-MAROTO, F.; ALVAREZ-BERMEJO, J. A.; MANZANO-AGUGLIARO, F.: DNA sequencing sensors: an overview. Sensors (Basel), v. 17, n. 3, p. 588, 2017. DOI: https://doi.org/10.3390/s17030588. PMID: 28335417; PMCID: PMC5375874.
NESTOR, C. E. et al.: Tissue type is a major modifier of the 5-hydroxymethylcytosine content of human genes. Genome Research, v. 22, p. 467-477, 2012. DOI: https://doi.org/10.1101/gr.126417.111.
PICELLI, S.: Single-cell RNA-sequencing: the future of genome biology is now. RNA Biology, v. 14, p. 637-650, 2017. DOI: https://doi.org/10.1080/15476286.2016.1201618.
PIDSLEY, R. et al.: Critical evaluation of the Illumina MethylationEPIC BeadChip microarray for whole-genome DNA methylation profiling. Genome Biology, v. 17, p. 208, 2016. DOI: https://doi.org/10.1186/s13059-016-1066-1.
RAMALHO-CARVALHO, J.; HENRIQUE, R.; JERÓNIMO, C.: Methylation-specific PCR. Methods in Molecular Biology, v. 1708, p. 447-472, 2018. DOI: https://doi.org/10.1007/978-1-4939-7481-8_23.
VARINLI, H. et al.: COBRA-Seq: sensitive and quantitative methylome profiling. Genes (Basel), v. 6, p. 1140-1163, 2015. DOI: https://doi.org/10.3390/genes6041140.
WANG, K. C.; CHANG, H. Y.: Epigenomics: technologies and applications. Circulation Research, v. 122, p. 1191-1199, 2018. DOI: https://doi.org/10.1161/CIRCRESAHA.118.310998.