Tópicos em Biologia Tumoral

Ementa

Abordagem de elementos relevantes da biologia tumoral, dentro de um enfoque celular e molecular, destacando-se aspectos relativos às células neoplásicas, ao microambiente tumoral e à resposta do hospedeiro, em associação aos principais métodos de investigação destes eventos. Circuitaria molecular no câncer: ciclo celular, proliferação e diferenciação celular. Imuno-histoquímica: princípios e aplicações, recuperação antigênica e sistemas de amplificação. Genética molecular do câncer: instabilidade cromossômica e mutações, oncogenes, genes supressores de tumores, eventos epigenéticos. Estroma e invasão tumoral: vasculogênese tumoral (contexto molecular, padrões de organização, marcadores e quantificação), MEC e câncer, hipóxia e progressão tumoral. Inflamação, macrófagos, células dendríticas, células natural-killer e câncer. Câncer como doença sistêmica/metástase.

Bibliografia

CHAKROBORTY, D.; SARKAR, C.; BASU, B.; DASGUPTA, P.S.; BASU, S. Catecholamines regulate tumor angiogenesis. Cancer Res., v. 69, n. 9, p. 3727-3730, 2009.
CHEN, J.J.W.; LIN, Y.C.; YAO, P.L.; YUAN, A.; CHEN, H.Y.; SHUN, C.T.; TSAI, M.F.; CHEN, C.H.; YANG, P.C. Tumor-associated macrophages: the double-edged sword in cancer progression. J Clin Oncol., v. 23, n. 5, p. 953-964, 2005.
DE WEVER, O.; MAREEL, M. Role of tissue stroma in cancer cell invasion. J Pathol., v. 200, n. 3, p. 429-447, 2003.
DIAZ-CANO S.J. General morphological and biological features of neoplasms: integration of molecular findings. Histopathology, v. 53, n. 1, p. 1-19, 2008.
FAVARO, E.; AMADORI, A.; INDRACCOLO, S. From tumor lymphangiogenesis to lymphovascular niche. APMIS, v. 116, p. 648-659, 2008.
FOX S.B.; HARRIS A.L. Histological quantitation of tumour angiogenesis. APMIS, v. 112, n. 7, p. 413-430, 2004.
FREIREICH E.J.; ESTROV Z.; KURZROCK R. Metastasis – an alternative hypothesis. Cancer, v. 103, n. 8, p. 1537-1539, 2005.
GUARINO, M. Epithelial-mesenchymal transition and tumour invasion. The International Journal of Biochemistry & Cell Biology, v. 39, n. 12, p. 2153-60, 2007.
GUPTA G.P.; MASSAGUÉ J. Cancer metastasis: building a framework. Cell, v. 127, n. 4, p. 679-695, 2006.
HAN, Z. et al. Epithelial-mesenchymal transition in tumor microenvironment. Cell & Bioscience, v. 1, p. 1-7, 2011.
HANAHAN, D.; WEINBERG, R.A. The hallmarks of cancer. Cell, v. 100, n. 1, p. 57-70, 2000.
HANAHAN, D.; WEINBERG, R.A. The hallmarks of cancer: the next generation. Cell, v. 144, n. 5, p. 646-674, 2011.
HICKLIN, D. J.; ELLIS, L.M. Role of the vascular endothelial growth factor pathway in tumor growth and angiogenesis. J Clin Oncol, v. 23, p. 1011-1027, 2005.
JING, Y.; HAN, Z.; ZHANG, S.; LIU, Y.; WEI, L. Epithelial-mesenchymal transition in tumor microenvironment. Cell & Biosc., 1:29, 2011.
JOYCE, J.A.; POLLARD, J.W. Microenvironmental regulation of metastasis. Nature Reviews Cancer, v. 9, n. 4, p. 239-252, 2009.
KLEIN, C.A. Parallel progression of primary tumours and metastasis. Nature Reviews Cancer, v. 9, n. 4, p. 302-312, 2009.
KROEMER, G.; POUYSSEGUR, J. Tumor Cell Metabolism: Cancer's Achilles´ Heel. Cancer Cell Review, v. 13, n. 6, p. 472-482, 2008.
LEONG, T.Y.; LEONG, A.S. How does antigen retrieval work? Advances in Anatomic Pathology, v. 14, n. 2, p. 129-131, 2007.
LEWIS, C.; MURDOCH, C. Macrophage responses to hypoxia-implications for tumor progression and anti-cancer therapies. The American Journal of Pathology, v. 167, n. 3, p. 627-35, 2005.
LOEB, L.A.; LOEB, K. R.; ANDERSON, J. P. Multiple mutations and cancer. PNAS, v.100, n. 3, p. 776-781,2003.
LOURO, I.D.; LLERENA JR, J.C.; MELO, M.S.V.; ASHTON-PROLLA, P.; CONFORTI-FROES, N. Genética molecular do câncer. São Paulo: MSG Produção Editorial, 2002.
MURPHY, M. E. Regulation of cell death in oncogenesis. Cancer Research, v. 65, p. 8069-8071, 2005.
PSAILA, B.; LYDEN, D. The metastatic niche: adapting the foreign soil. Nat Rev Cancer, v. 9, p. 285-293, 2009.
RAMOS-VARA, J.A. Technical aspects of immunohistochemistry. Veterinary pathology, v. 42, n. 4, p. 405-426, 2005.
RIBATTI, D. et al. The structure of the vascular network of tumors. Cancer Letters, v. 248, n. 1, p. 18-23, 2007.
RUEGG, C. Leukocytes, inflammation, and angiogenesis in cancer: fatal attractions. Journal of Leukocyte Biology, v. 80, n. 4, p. 682-4, 2006.
SCAVELLI, C. et al. Lymphatics at the crossroads of angiogenesis and lymphangiogenesis. Journal of anatomy, v. 204, n. 6, p. 433-449, 2004.
TLSTY, T.D.; COUSSENS, L. M. Tumor stroma and regulation of cancer development. Annu Rev Pathol Mech Dis , v. 1, p. 119-50, 2006.
VERMEULEN, K.; VAN BOCKSTAELE, D.R.; BERNEMAN, Z.N. The cell cycle: a review, deregulation and therapeutic targets in cancer. Cell Proliferation, v. 36, p. 131-149, 2003.
WEBSTER, J.D. et al. Cellular proliferation in canine cutaneous mast cell tumors: associations with c-KIT and its role in prognostication. Veterinary Pathology, v. 44, n. 3, p. 298-308, 2007.
WEGROWSKI, Y.; MAQUART, F. X. Chondroitin sulfate proteoglycans in tumor progression. Adv Pharmacol., v. 53, p. 297-321, 2006.
WEINBERG, R.A. A biologia do câncer. Porto Alegre: Artmed, 2008.