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Laboratory of Cellular and Molecular Biology

 

PRESENTATION

The Universidade Paulista has efficient administrative infrastructure that provides all the support for typing, teaching organization, monitoring of deadlines for filing dissertations and other bureaucratic activities. The UNIP structure now has research laboratories that support Graduate level activities Masters and PhD. All projects for Master's or PhD dissertation are experimental in nature. To do so, students have the following facilities:

  • Laboratory of Molecular and Cellular Biology with cutting edge equipment that allow to develop projects that will result in competitive international publications. It is estimated that, in addition to projects of a basic nature, as the influence of epigenetic factors in the differentiation of B - 1 cells, molecular biology is used in other subjects as already in development by some teachers. The investment of approximately R$ 800,000.00 in the construction of the laboratory came undoubtedly facilitate the update of the levels of research developed in the institution. The laboratory occupies an area of approximately 200 m² divided into three sub-areas, namely; Cell Biology, Molecular Biology and Infectious Agents. Apart from consumables, countertops and other basic facilities, the laboratory has equipment such as; autoclaves, analytical balance, cabin class II - A1 for manipulating microorganisms, digital camera Fotodoc gel excelsa centrifuge, baby refrigerated centrifuge, microscope trinocular microscope phase contrast and fluorescence, bacteriological incubator, sterilizer, incubator CO², Transsidelight Photo / UV 21, source for electrophoresis, upright freezer, Hood with LCD Display for Fotodoc Gel, horizontal minicuba for electrophoresis, Osmosis reverse and Mili - Q, Thermocyclers, Fax and other facilities.
  • The Laboratory of Pathology, Institute of Health UNIP has all the facilities for studies in optical microscopy as stoves, refrigerators, microtomes, microscope image analysis and others. In association with this laboratory are being implemented methods and techniques in Immunohistochemistry, in association with the Molecular Biology, will be of great value to the development of several projects that require these methodologies. If necessary the use of more advanced and expensive techniques such as electron microscopy, confocal microscopy and other agreements with institutions that have such services shall be established until appropriate purchase such equipment from UNIP or funding agencies.
  • It also offers laboratory that aims to extract active ingredients from plants of the Amazon and Atlantic forest with therapeutic properties in vitro and in vivo experimental models.
  • Finally, is under construction experimental animal facility for small rodents at the Laboratory of Cellular and Molecular Biology. Along with this vivarium is also being built laboratory for behavioral studies with rodents, thus occasioning the investigation of issues related to neuroscience.

INSTITUTIONAL PARTNERSHIPS / EXCHANGES / COVENANTS

All teachers of the course maintain partnerships with other educational institutions and/or research, developing projects together and enabling our MSc students and undergraduate research accomplishment and apprehension methodologies/techniques used in these locations. The information below illustrates this type of activity.

Partnerships

Vania Maria de Carvalho, Instituto Adolfo Lutz Institute and Biomedical Sciences - USP. Genotypic and phenotypic characterization of samples of uropathogenic Escherichia coli (UPEC) isolated from urinary tract infections (UTI) in dogs and cats. Research Lecturer

Vania Maria de Carvalho, Institute of Biomedical Sciences - USPHOVET - USP Instituto Adolfo Lutz and Diagnostic Lab & Vet and Veterinary Consulting, John Carlos B. Santos Filho / Renata de Oliveira Iovine. Search virulence factors and drug sensitivity of Escherichia coli from clinical cases of canine pyometra. Master / Scientific Initiation

Vania Maria de Carvalho Park Zoo Foundation of São Paulo, Luciana Gallo War, Search Salmonella in wild canids. Scientific Initiation

Vania Maria de Carvalho, Lab & Vet Veterinary Diagnostic and Consulting, Natália Ferreira Ramalho, Characterization of samples of Proteus mirabilis isolated from otitis in dogs. Scientific Initiation.

Vania Maria de Carvalho, Lab & Vet Veterinary Diagnostic and Consulting, Karolyne Lombardi Moraes, Characterization of samples of Proteus spp. isolated from urinary tract infection in dogs. Scientific Initiation

Selene Dall ' Acqua Coutinho, Instituto Adolfo Lutz, Graziela Habib Nardi, Malassezia spp. in the external auditory meatus of dogs with and without otitis : phenotypic and genotypic characterization. master's degree

Selene Dall ' Acqua Coutinho, Adolfo Lutz Institute, Zoological Park of São Paulo Foundation and the Working Group on Conservation of anteater - Brazil (GTCT). Henri D. Bentubo, Search dermatophytes and Malassezia spp. in the haircoat of anteaters. improvement

Selene Dall ' Acqua Coutinho Foundation Zoological Park of São Paulo Henrique Paloma Canedo Malassezia spp. in the external auditory canal and fur of wild canids. Scientific Initiation

Selene Dall ' Acqua Coutinho Foundation Zoological Park of São Paulo Pimentel Sandara Sguario. Search dermatophytes on the haircoat of wild canids. Scientific Initiation

Maria Anete Lallo,Laboratory of Immunology,Federal University of São Paulo (UNIFESP), Adriano Pereira, Immunohistochemical identification of cellular components of the inflammatory infiltrate in tissues experimentally infected by Encephalitizoon cuniculi in Balb / C mice treated with cyclophosphamide. Master.

Maria Anete Lallo, School of Public Health, Diagnosis of Encephalitozoon cuniculi encephalitozoonosis and Cryptosporidium by nested PCR technique. Research Professor.

V. Leoni Bonamin, Laboratory of Immunology  Federal University of São Paulo(UNIFESP) Ruggero Zalla Neto, immune to BCG in mice treated with homeopathic thymulin Answer. master's degree

Lucia Jamli Abel, Laboratory of Immunology, Federal University of São Paulo (UNIFESP) and Wallaceville Research CentreAgResearch Farming Food and Health, New Zealand, Priscilla M. DeNapoli, Evaluation of humoral and cellular immune response in sheep treated with immunostimulatory associated with anthelmintic. Master.

Lucia Jamli Abel, Laboratory of Immunology, Federal University of São Paulo (UNIFESP), Effect of combined use of immunostimulatory the anthelmintic in experimental sheep worms by detecting serum IgE. Research Lecturer

Lucia Jamli Abel, Laboratory of Immunology, Federal University of São Paulo (UNIFESP) and Department of Infectious and Parasitic Diseases, State University of São Paulo (UNESP - Jaboticabal), Chen Shelllen Sartorello. Immunostimulatory effect associated with anthelmintic in immune sheep infected with Haemonchus contortus response. Scientific Initiation

Eduardo F. Bondan, College of Veterinary Medicine and Animal Science, USP (Immunohistochemistry Laboratory, Laboratory of Transmission Electron Microscopy, Department of Pathology) and Federal University of Santa Maria (Laboratory of Transmission Electron Microscopy). Marking the astrocytic response Immunohistochemistry expression of GFAP (glial fibrillary acidic protein) and vimentin in the central nervous system (CNS) in dogs with distemper. Research Lecturer

Maria Martha Bernardi,College of Veterinary Medicine and Animal Science of USP. Luiz Renato Flaquer. Evaluation of anti-inflammatory and antinociceptive effects of polar and nonpolar extracts of Nepeta cataria. Master.

Mario Mariano, Laboratory of Immunology, Federal University of São Paulo (UNIFESP) Maria Carolina Andrade. Involvement of B - 1 cells with concomitant tumor immunity in mice Erlich. Master.

Exchanges

The Professor Dr. Vania Maria de Carvalho exchange began in 2007 with the "National Zoological Cuba" Havana, in order to assist the researcher Jorge Polo in the characterization of E. coli isolates from wildlife in Cuba.

Professor Dr Lucia Jamli Abel maintains exchange since 2006 with the Laboratory of Professor Dr. Richard Shaw of Wallaceville Institute in New Zealand, to carry out projects in the area of immune response in ruminants. One of the projects included in this partnership is developed by the student in the Master Priscilla DeNapoli.

Agreement

Recently the UNIP and the Syrian-Lebanese Hospital signed an agreement aimed at broad scientific exchange between the two institutions. Initially, the agreement focuses on the study of active ingredients from Brazilian plants. However, further opens the opportunity for cooperation in research in several areas of biomedicine considering the excellence of the Syrian-Lebanese Hospital researchers as well as modern technological features offered by this institution. Undoubtedly, the relationship between students and teachers of graduate programs in the area of ​​UNIP Health researchers with the Syrian-Lebanese is promising.

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